R.M Rivera, L. Bonilla, A.M. Brad, J.L. Edwards, A.D. Ealy, V.M. Monterroso,
A.C. Majewski, C.M. Franco, J.M. Moss, J.M. Fear, and P.J. Hansen
Dept. of Animal Sciences, University of Florida

The procedures for in vitro production [IVP; i.e. in vitro maturation (IVM), in vitro fertilization (IVF), and in vitro culture (IVC)] of embryos described here are based on procedures developed in other laboratories at the University of Wisconsin Xu et al., 1992), and University of Missouri (Edwards et al., 1997; Paula-Lopes et al., 1998).  Keep in mind that the protocols described here are not fixed but rather constantly evolve as new developments take place.  Therefore, practitioners of IVP will be well advised to experiment with the procedures used, especially after reading of improvements made by other laboratories.  This protocol is organized by day of the protocol with d 0 being the day of fertilization.

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DAY-BY-DAY PROCEDURE
Abbreviated Protocol for the Experienced User
Instructions for Printing This Manual        Obtaining The Manual in PDF Format

Abbreviated Protocol in Spanish (thanks Adriana Fernandez)

Detailed Protocol in Spanish (protocol by Luis Luis Armando Dávila Félix - thanks Luis)

Abbreviated Protocol in Portuguese (thanks Rodrigo Nunes and Luiz de Castro e Paula)

OTHER INFORMATION

Click on the icon to see recent changes in the IVP Protocol

Do you want a hard copy of our embryo poster? Drop a line by email to Peter Hansen or Sabrina Robinson with your shipping address and we will send you at no charge an 8.5" x 11" color poster illustrating all of the developmental endpoints observed during IVP of bovine embryos. 

Contact the Author:    Hansen.  We want your feedback!! Let us know whether this protocol works for you.