OTHER PROTOCOLS USEFUL FOR WORKING

WITH OOCYTES AND EMBRYOS

Alternative sperm purification protocols - tired of Percoll?  here are some other protocols for purifying sperm.

Alternative oocyte processing procedure – this is the original procedure placed on the website.

Cryopreservation:  Slow Freezing |  Vitrification (Seidel)  | Open-pulled straw (Vajta)Open-pulled straw (Vajta-Spanish) 

Determining lipid content in embryos using Nile Red fluorescence

Differential staining of trophectoderm and inner cell mass in combination with TUNEL labeling of bovine embryos

Fertilization in Drops - for fertilizing in microdrops

Immunolocalization of CDX2

In Vitro Fertilization with Sex-Sorted Semen

Searching for oocytes - the Movie! 

Zona pellucida removal

this page was last updated  8-12-11

all original material for this website © Rocio Rivera, Peter J. Hansen et al. 2000-2011

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