OTHER PROTOCOLS USEFUL FOR WORKING

WITH OOCYTES AND EMBRYOS

Alternative sperm purification protocols - tired of Percoll?  here are some other protocols for purifying sperm.

Alternative oocyte processing procedure – this is the original procedure placed on the website.

Caspase 8 and 9 binding assay for embryos

Cell number counting in preimplantation embryos

Cryopreservation: Slow Freezing | Vitrification

Cumulus removal using hyaluronidase

Differential staining of trophectoderm and inner cell mass in combination with TUNEL labeling of bovine embryos

Preparation of in vitro derived bovine embryos for transfer into recipients

Glutathione assay - a microassay for determining glutathione content in cells, optimized for use in embryos

Guide on use of instruments for picking up oocytes and embryos

Hemacytometer instructions - this procedure can be used for counting sperm.

In vitro fertilization with sexed semen

Mini-Chamber for regulating gaseous environment during culture

Differential staining of trophectoderm and inner cell mass in combination with TUNEL labeling of bovine embryos

TUNEL procedure for preimplantation embryos

Zona pellucida removal with Pronase

 

 

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this page was last updated  7-24-07

all original material for this website © Rocio Rivera, Peter J. Hansen et al. 2000-2002

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