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IVF MEDIA – RESPONSIBILITIES AND SCHEDULE
The primary person assigned to make
media will be expected to make all TALPs and other selected media and stocks no
later than Monday morning on the week assigned. The seconday person will be
expected to replenish all supplements that are running low by Friday of the
week assigned. Enough of each media and
supplement should be available for the entire week’s IVF runs. The person assigned for cleaning will have
responsibility to ensure that the IVF culture room is clean and that inventory
of supplies for IVF is adequate.
A logbook of culture media and cleaning
is maintained. Please use it to record each time a new medium or medium
supplement is made.
Logsheets can be
downloaded if needed and stored in the file in the culture room.
Specific
media and supplements assigned to each person are as follows:
Primary
OCM (oocyte collection medium)
OMM (oocyte maturation medium)
90% Percoll
HEPES-TALP
IVF-TALP
10X Sp-TL
Estradiol
Na-Pyruvate stock
MgCl2
CaCl2
Saline (if dishwasher has not made it)
Fill out checklist in the IVF Media and Cleaning
Log
Secondary
PHE (penicillamine/hypotaurine/epinephrine)
BSS (bovine steer serum)
BSS/Heparin (bovine steer serum+heparin)
Folltropin
Heparin
Gentamicin 10 ul and 1 ml
Glutamine 1 ml and 4 ml
Hyaluronidase
Pen/strep (penicillin/streptomycin) 4 ml
Fill out checklist in the IVF Media and Cleaning
Log
Cleaning
Mop floor with 10% bleach
Throw out large biohazard bag in outer culture room and replace
Change water bath
Wipe the surface of the hood with ethanol
Wipe the inside of the centrifuge with ethanol
Wipe the benches, refrigerator and incubator tops
and other horizontal surfaces with ethanol
Wipe the refrigerator door and seal on the refrigerator door with
ethanol
Clean the incubator doors with 70% ethanol
First Week of Month: Change water in incubator pan – add piece
of copper to each pan
Make sure nothing is stored in hoods and little is stored on benches
Check to make sure inventory is adequate - pipette tips, dishes, filters, reagents, etc - everything
Fill out checklist in the IVF Media and Cleaning
Log
In addition, the person assigned to maintain
incubators should do the following each week
Check CO2 and O2 levels with fyrite
Check whether temperature in each incubator is
accurate
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