RECIPES FOR MEDIA
PREPARED BY CAISSON
TL-HEPES | TL-Fert (IVF-TL) | TL-Sperm | KSOM+AA
IVP
Home Page
Currently, we use Caisson as our supplier for KSOM and TL
media. These are custom-made for us and
probably can also be ordered by others.
The medium
referred to here as Hepes-TL is labeled
TL-HEPES by Caisson, the medium labeled IVF-TL is called TL-Fert
by Caisson and the medium Sp-TL is called TL-Sperm by Caisson. There are no
catalog numbers.
|
TL-HEPES (i..e., HEPES-TL) |
|||
|
Volume |
|
500 ml |
|
|
Osmolarity |
|
260-270 |
|
|
pH |
|
7.3-7.4 |
|
|
Component |
Mol Wt/Form Wt |
mM |
mg/L |
|
NaCl |
58.5 |
114.0 |
6669.0 |
|
KCl |
74.6 |
3.2 |
238.72 |
|
NaHCO3 |
84.0 |
2.0 |
168.0 |
|
NaH2PO4*H2O |
120.0 |
0.4 |
48.0 |
|
Na Lactate (792 g/L) |
112.1 |
10.0 |
1.416 ml |
|
CaCl2 |
110.1 |
2.0 |
220.2 |
|
MgCl*6H2O |
167.8 |
0.5 |
83.9 |
|
HEPES |
238.4 |
10.0 |
2384.0 |
|
TL-Fert |
|||
|
Volume |
|
100 ml |
|
|
Osmolarity |
|
280-300 |
|
|
pH |
|
7.3-7.4 |
|
|
Component |
Mol Wt/Form Wt |
mM |
mg/L |
|
NaCl |
58.5 |
114.0 |
6669.0 |
|
KCl |
74.5 |
3.2 |
238.4 |
|
NaHCO3 |
84.0 |
25.0 |
2100.0 |
|
NaH2PO4*H2O |
138.0 |
0.4 |
55.2 |
|
Na Lactate (792 g/L) |
112.1 |
10.0 |
1.416 ml |
|
CaCl2 |
111.1 |
2.0 |
222.2 |
|
MgCl*6H2O |
203.3 |
0.5 |
101.65 |
|
Volume |
|
100 ml |
|
|
Osmolarity |
|
290-300 |
|
|
pH |
|
7.3-7.4 |
|
|
Component |
Mol Wt/Form Wt |
mM |
Mg/L |
|
NaCl |
58.5 |
100.0 |
5850.0 |
|
KCl |
74.5 |
3.1 |
230.95 |
|
NaHCO3 |
84.0 |
25.0 |
2100.0 |
|
NaH2PO4*H2O |
138.0 |
0.29 |
40.02 |
|
Na Lactate (792 g/L) |
112.1 |
26.0 |
3.68 ml |
|
CaCl2*2H2O |
147.1 |
2.107 |
310 |
|
MgCl*6H2O |
203.3 |
0.4 |
81.32 |
|
HEPES |
238.3 |
10.0 |
2383.0 |
|
Volume |
100 ml |
|
Osmolarity |
|
|
pH |
7.3-7.4 |
|
Component |
mg/L |
|
CaCl2*2H2O |
250.0 |
|
KCl |
186.38 |
|
KH2PO4 |
47.99 |
|
MgSO4*7H2O |
49.3 |
|
NaCl |
5551.8 |
|
NaHCO3 |
2100.25 |
|
Na lactate syrup |
1.415 ml 1121.00 mg/L |
|
Na Pyruvate |
22.0 |
|
EDTA |
3.72 |
|
D-glucose |
36.032 |
|
L-glutamine |
146.15 |
|
L-Arginine HCl |
63.2 |
|
L-Cystine |
12.02 |
|
L-Histidine HCl H2O |
20.96 |
|
L-Isoleucine |
26.23 |
|
L-Leucine |
26.24 |
|
L-Lysine HCl |
36.52 |
|
L-Methionine |
7.46 |
|
L-Phenylalanine |
16.52 |
|
L-Threonine |
23.82 |
|
L-Tryptophan |
5.11 |
|
L-Tyrosine |
18.12 |
|
L-Valine |
23.42 |
|
L-Serine |
5.26 |
|
L-Alanine |
4.45 |
|
L-Asparagine H2O |
7.5 |
|
L-Aspartic Acid |
6.66 |
|
L-Glutamic Acid |
7.36 |
|
L-Proline |
5.76 |
|
BSA |
1000 |
|
Glycine |
3.75 |
this page was last
updated 5-19-05
all original material for this website
© Rocio Rivera, Peter J. Hansen et al. 2000-2004
Links to commercial sites do
not constitute endorsement by the authors or the
