1. Pre-warm stage of inverted microscope by placing heater near the microscope for at least 15 min prior to use.

2. Assess cleavage rate of embryos by determining the number of embryos cleaved divided by the number of embryos/oocytes placed initially in the microdrops.  Return plates to the incubator.

1.  Place aliquots of heat-inactivated fetal calf serum (stock 24) in the incubator ~1-2 h before adding to the microdrops containing the embryos/oocytes.

2. Pre-warm air by placing the heater in front of the slide warmer.

3. Place plates on the slide warmer and add 5 ml of sterile filtered heat treated fetal calf serum to each microdrop and return to incubator.

1. Pre-warm stage of inverted microscope by placing heater near the microscope for at least 15
      min prior to use.

2.   Assess development of embryos to the blastocyst stage.  Return plates to the incubator.

Click here for description of embryos at different stages.

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