DISHWASHING
Soap solutions |
Dishwashing | Autoclaving
IVP
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Soap bath
(to wash glassware, and instruments)
Dilute PCC concentrate in deionized water according to manufacturer's instructions
7X Cleaning solution (ICN) – fill a
squirt bottle for ease of use
Dishwashing
Dirty dishes are rinsed in water,
any tape immediately removed, and soaked in soap bath until dishes are
done. Do not leave dirty dishes to air dry and do not leave them with
traces of medium inside.
Glassware, caps, collection medium carboy, stir bars,
and instruments
1) remove any tape/label
left on the bottle/caps (this
should have been done beforehand)
2) fill bottle/beaker with some dilute
3) clean with brush
4) rinse 5X with warm/hot tap water (make sure there
is no soap left)
5) rinse 5X with deionized water
6) rinse 1X with distilled/deionized water
7) Let air dry
Plastic beakers/containers
1) squirt some 7X cleaning solution into the
container
2) wash with brush or sponge
3) rinse 5X with warm/hot tap water (make sure
there is no soap left)
4) rinse 5X with deionized water
5) Let air dry
Autoclaving
after dishes are completely
dried
Bottles/ collection medium carboy
place cap loosely onto the bottle and put a
piece of autoclave tape.
Stir bars/ instruments
place inside Tower
DualPeel® autoclave tubing and place
autoclave tape at both ends.
Alternatively, heat-seal the ends using an impulse sealer (Agtech). Bars will turn black when autoclaved.
Beakers/volumetric flasks
Cover snugly with a double layer of aluminum
foil. Place a piece of autoclave tape on the foil.
Autoclave for a minimum of 25 minutes and dry cycle 15 minutes. After autoclaving is done, tighten bottle caps a little bit to prevent any contaminants to enter. Do not tighten caps completely until bottles have cooled to prevent a vacuum forming in the bottle.
this page was last
updated 05-10-05
all original material for
this website © Rocio Rivera, Peter J. Hansen et al. 2000-2002
Links to commercial sites do
not constitute endorsement by the authors or the
