What's New - UF Bovine IVP

What's New In IVP
This page will outline specific changes to the IVP protocol.

12-15-09. Added a pdf file for the Day -1 protocol.

12-15-09. Added a video page.

12-15-09. Added a movie made by Luis Davila of Luciano Bonilla slashing ovaries on the Day -1 webpage. - click here

10-04-09. Embedded the movie showing searching for oocytes on the Day -1 webpage

10-02-09. A movie showing searching for oocytes has been added - click here

09-30-09. Changed the recipe for OMM to use Glutamax instead of glutamine. Made minor modifications on the gentamicin stock formulation (stock 8).

09-29-09. The recipe for OMM has been changed to reduce the volume made up each day. Changed the recipes for Stocks 3 and 8 to reflect the changes to OMM. Also, created a pdf file for stock solutions.

09-25-09. Made additional changes to the SOF recipe and minor maintenance to some files.

07-29-09. Changed the SOF recipe.

04-10-09. Added a protocol for use of sexed semen and more pdf files.

03-31-09. Beginning a process to convert pages to pdf files to make it easier to print and read. This will take a lot of time. PDF files can be obtained here.

03-31-09. Changed the fertilization procedure to use dishes (200 oocytes per dish) rather than drops. It is easier, gives similar results, and cheaper. The old procedure for fertilization in drops is available.

03-24-09. Changed the catalog number for the Millipore formulation for SOF (BSS-077-D; replaces BSS-046-D).

01-21-08. Changes some details regarding suppliers of medium (Biowhittaker is now Lanza and Specialty Media is now Millipore).

7-24-07. Added a protocol for use of sexed semen and for zona pellucida removal.

7-20-07. Added a new, detailed protocol in Spanish prepared by Luis Davila.

2-17-06. An abbreviated protocol in Spanish prepared by Adriana Fernandez has now been added. Also, the list of suppliers has been updated.

1-13-06. The old dps.ufl.edu server has been taken down. Some old links in the website to www.dps.ufl.edu are no longer functional. We are trying to identify these. If you come to a blind link, please replace dps with animal in the address and the page will open.

5-19-05. We have changed the description of Stock 1 to reflect the fact we purchase lactate syrup as a 98% syrup.

5-19-05. We have identified a typo in the description of the PHE solution. The epinephrine stock is actually 250 mM and not 250 mM. The formulation is correct as always written. We have also clarified the description of the procedure to make PHE.

2-10-05. The procedure for diluting sperm has been changed to correct an error in the concentration of sperm prepared after purification by Percoll (see day 0).

9-1-04. New recipes for culture media prepared by Caisson Labs have been added – much cheaper than other supplies and of high quality!

1-21-04. The procedure for processing oocytes after slashing has undergone a major change involving use of a cell strainer to simplify the procedure (-see day -1). The old procedure has been retained on a separate page.

7-9-02. We are in the process of editing the entire protocol for typos and changes to the procedure that have been made since the protocol was originally placed online in April, 2000. As each page is changed, it is being given a new look to distinguish it from the older pages.

5-1-01.We are making available hard copies of our embryo poster. Drop us a line by email with your shipping address and we will send you at no charge an 8.5" x 11" color poster illustrating all of the developmental endpoints observed during IVP of bovine embryos. If you would like a more elaborate poster, we will be happy to make special arrangements to do so.

4-2-01. Photographs of bovine embryos produced by IVP was added (see Embryo Gallery).

2-20-01. Link to new protocol for counting cell number in embryos was added (see Other Embryo Protocols).

IVP Home Page

this page was last updated 7-24-06

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