Laboratory of P.J. Hansen

        LIST OF PROTOCOLS - P.J. HANSEN LAB 

Assays |  Embryos |  Immunochemical Procedures  |  Molecular Biology  |  Tissue and Cell Culture
Other Procedures  |  Other Links

        Interested in other protocols than those listed here?  Search the database from Protocol Online database using the box below!!

        ASSAYS

          Glutathione - a microassay for determining glutathione content in cells

Bradford Protein Assay  - a microassay for determining protein content in a 96-well microtiter plate format

Lowry Protein Assay

        EMBRYOS  

Apoptosis

FAM caspase 8 and 9 binding assay for embryos

Group II caspase activity in bovine embryos

TUNEL procedure for preimplantation embryos

Cell Counting

Cell number counting in preimplantation embryos

 

Live/dead stain for embryos and oocytes

 

Differential staining of trophectoderm and inner cell mass of bovine embryos

Cryopreservation: Slow Freezing | Vitrification

In Vitro Production

In Vitro Production of Bovine Embryos (note the link on the index at this site for other embryo protocols)

Other

Detection of reactive oxygen species in preimplantation bovine embryos

Mini-Chamber for regulating gaseous environment during culture
 

        IMMUNOCHEMICAL PROCEDURES

Immunoprecipitation

 

Western blotting using ECL reagent

       

        MOLECULAR BIOLOGY

         RNA purification using TRizol    

        TISSUE AND CELL CULTURE

Culture of endometrial explants
 

Culture of BEND cells (a bovine endometrial cell line)
 

Hemacytometer, Use of

      OTHER PROCEDURES

      OTHER LINKS

Apoptosis Protocols – part of the Immunohistochemistry World Website     

Buffer Design  For those who have trouble designing buffers and calculating molarity, here is the site for you (from Professor Rob Benyon at University of Manchester

 

Crown rump length - use this site from John Parrish to calculate stage of gestation in cattle

Fixation Procedures for Flow Cytometry – From the University of Iowa, these work for immunocytochemistry too.

          Histotechnology (Univ. Nottingham)

          Immunohistochemistry Protocols from Immunohistochemistry World   

         Immunoportal - everything immunohistochemical

Molecular Biology Protocols 

Online Resources for Immunology Research Using Comparative and Veterinary Animal Models

Ovine Formulary  A list of protocols for inducing anesthesia in sheep

 

Power Calculations - from R.V. Lenth, University of Iowa

Protocols Online  An excellent source of protocols for biological research

University of Florida Flow Cytometry Core Lab – protocols, software and links

UNC Tissue Culture Facility - a great site, includes some tips on recognizing and dealing with contamination

Understanding and Managing Cell Culture Contamination - from Corning

University of Pennsylvania School of Dental Medicine Flow Cytometry Facility

WWW Virtual Library of Cell Biology - Methods, Protocols, and Software A good collection of links to lab protocols