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LIST OF PROTOCOLS
- P.J. HANSEN LAB 
Assays |
Embryos |
Immunochemical Procedures |
Molecular Biology |
Tissue and Cell Culture
Other Procedures |
Other Links
Interested in other protocols than those listed
here? Search the database from Protocol Online database using the box
below!!
 ASSAYS
Glutathione - a microassay for determining glutathione content in cells
Bradford Protein Assay - a microassay for determining protein content in a
96-well microtiter plate format
Lowry Protein Assay
EMBRYOS
Apoptosis
FAM caspase 8 and 9 binding assay for embryos
Group II
caspase activity in bovine embryos
TUNEL procedure for preimplantation embryos
-
see
Tutorial ImageJ for Luciano Bonilla's guide for using ImageJ for counting
cell number.
Cell Counting
Cell number counting in preimplantation embryos
Live/dead stain for embryos and oocytes
Differential staining of trophectoderm
and inner cell mass of bovine embryos - see
Tutorial ImageJ for Luciano Bonilla's guide for using ImageJ for counting
cell number.
Cryopreservation:
Slow Freezing |
Vitrification
In Vitro Production
In Vitro Production of Bovine Embryos (note the link on the index at this
site for other embryo protocols)
Other
Detection of reactive oxygen species in preimplantation bovine embryos
Determining lipid content in embryos using Nile Red fluorescence
Immunodetection of 5-methylcytosine in preimplantation embryos
Mini-Chamber for regulating gaseous environment during culture
Searching for
oocytes - the Movie!
IMMUNOCHEMICAL
PROCEDURES
Antigen retrieval for immunohistochemistry with
paraffin-embedded tissues
Immunoprecipitation
Western blotting using ECL reagent
MOLECULAR
BIOLOGY
RNA purification using TRizol
TISSUE
AND CELL CULTURE
Culture of endometrial explants
Culture of BEND cells (a bovine endometrial cell line)
Hemacytometer, Use of
OTHER
PROCEDURES
Acrosomal stain
pH Meter (Accumet X15):
Protocol for use in PJH lab
Manual
Rectal palpation of
cattle
Scintillation
spectrometry - Manual for Beckman LSC-6000
Synthesis of
glutathione ester
OTHER LINKS
Apoptosis Protocols – part of the Immunohistochemistry World Website
Buffer Design
For those who have
trouble designing buffers and calculating molarity, here is the site for you
(from Professor Rob Benyon at University of Manchester
Crown rump length - use this site from John Parrish to calculate stage of
gestation in cattle
Fixation Procedures for Flow Cytometry – From the University of Iowa, these
work for immunocytochemistry too.
Histotechnology (Univ. Nottingham)
Immunohistochemistry Protocols from Immunohistochemistry
World
Immunoportal - everything immunohistochemical
Molecular Biology Protocols
Online Resources for Immunology Research Using Comparative and Veterinary Animal
Models
Open Wetware
- From MIT, the site "is
an effort to promote the sharing of information, know-how, and wisdom among
researchers and groups who are working in biology & biological engineering".
Ovine Formulary A list of protocols for inducing anesthesia in sheep
Power Calculations - from R.V. Lenth, University of Iowa
Protocols Online An excellent source of protocols for biological research
University of Florida Flow Cytometry Core Lab – protocols, software and
links
UNC Tissue Culture Facility - a great site, includes some tips on
recognizing and dealing with contamination
Understanding and Managing Cell Culture Contamination - from Corning
University of Pennsylvania School of Dental Medicine Flow Cytometry Facility
WWW Virtual Library of Cell Biology - Methods, Protocols, and Software A
good collection of links to lab protocols
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