P.J. Hansen Home Page

       PJ Hansen Main Page                             Animal Sciences Home Page                              University of Florida

    LIST OF PROTOCOLS - P.J. HANSEN LAB 

Assays |  Embryos |  Immunochemical Procedures  |  Molecular Biology  |  Tissue and Cell Culture
Other Procedures  |  Other Links

        Interested in other protocols than those listed here?  Search the database from Protocol Online database using the box below!!

        ASSAYS

       Glutathione - a microassay for determining glutathione content in cells
       Bradford Protein Assay  - a microassay for determining protein content in a 96-well microtiter plate format
       Lowry Protein Assay

        EMBRYOS - PROCEDURES FOR WORKING WITH PREIMPLANTATION EMBRYOS

       Group II Caspase Activity in Bovine Embryos

       In Vitro Production of Bovine Embryos (note the link on the index at this site for other embryo protocols)
       Mini-Chamber for regulating gaseous environment during culture
       Cell number counting in preimplantation embryos

       Live/Dead stain for embryos and oocytes
       TUNEL procedure for preimplantation embryos

        IMMUNOCHEMICAL PROCEDURES

       

        MOLECULAR BIOLOGY

       RNA purification using TRizol    

        TISSUE AND CELL CULTURE

       Culture of endometrial explants
       Culture of BEND cells (a bovine endometrial cell line)
       Hemacytometer, Use of

      OTHER PROCEDURES

       Acrosomal stain
       Rectal palpation of cattle
       Synthesis of glutathione ester

      OTHER LINKS

       Apoptosis Protocols – part of the Immunohistochemistry World Website     

       Buffer Design  For those who have trouble designing buffers and calculating molarity, here is the site for you

         from Professor Rob Benyon at University of Manchester

       Fixation Procedures for Flow Cytometry – From the University of Iowa, these work for immunocytochemistry

        too.

       Histotechnology (Univ. Nottingham)

       Immunohistochemistry Protocols from Immunohistochemistry World      

       Molecular Biology Protocols 

       Online Resources for Immunology Research Using Comparative and Veterinary Animal Models

       Ovine Formulary  A list of protocols for inducing anesthesia in sheep
       Protocols Online  An excellent source of protocols for biological research

       University of Florida Flow Cytometry Core Lab – protocols, software and links

       University of Pennsylvania School of Dental Medicine Flow Cytometry Facility

       WWW Virtual Library of Cell Biology - Methods, Protocols, and Software A good collection of links to lab   

       protocols